help button home button
AJRCCM
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by MACFARLANE, A. J.
Right arrow Articles by BARNES, N. C.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by MACFARLANE, A. J.
Right arrow Articles by BARNES, N. C.

Am. J. Respir. Crit. Care Med., Volume 161, Number 5, May 2000, 1553-1558

Sputum Cysteinyl Leukotrienes Increase 24 Hours after Allergen Inhalation in Atopic Asthmatics

ALISON J. MACFARLANE, RYSZARD DWORSKI, JAMES R. SHELLER, IAN D. PAVORD, A. BARRY KAY, and NEIL C. BARNES

The London Chest Hospital, London, United Kingdom; Vanderbilt University, School of Medicine, Nashville, Tennessee; Department of Respiratory Medicine, Glenfield Hospital, Leicester, United Kingdom; and Allergy and Clinical Immunology, Imperial College School of Medicine, National Heart and Lung Institute, London, United Kingdom

We have used the relatively noninvasive technique of induced sputum to measure allergen-induced changes in the concentration of eicosanoid mediators in bronchial secretions from atopic asthmatics. Sputum induction was performed before and 24 h after inhalational allergen challenge in 14 atopic asthmatics who developed a late asthmatic reaction (LAR). Differential cell counts were made on sputum cytospins and eicosanoid (cysteinyl leukotrienes [cys LTs], prostaglandin D2 [PGD2], and PGE2) concentrations were measured in the sputum supernatants. The percentage of eosinophils at baseline correlated with the concentration of cys LTs (r = 0.84, p < 0.001) but not prostanoid mediators. Allergen challenge produced a significant increase in the concentration of sputum cys LTs from 3.45 ng/ml sputum to 11.95 ng/ml (p = 0.002), which correlated with the increase in sputum eosinophils (r = 0.55, p < 0.05). There were no significant changes in PGD2 or PGE2 concentrations in sputum supernatants in response to challenge. Thus, the noninvasive technique of induced sputum has been used to demonstrate increased cys LTs, but not prostanoids associated with LAR after allergen challenge. The correlation between eosinophil numbers and cys LT concentrations at baseline values and 24 h after allergen challenge is consistent with these cells being a principal source of cys LTs within the airways at these time points.




This article has been cited by other articles:


Home page
 Eur Respir JHome page
E. Ono, M. Taniguchi, H. Mita, N. Higashi, Y. Fukutomi, H. Tanimoto, K. Sekiya, C. Oshikata, T. Tsuburai, N. Tsurikisawa, et al.
Increased urinary leukotriene E4 concentration in patients with eosinophilic pneumonia
Eur. Respir. J., August 1, 2008; 32(2): 437 - 442.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
C. Thompson, A. Cloutier, Y. Bosse, C. Poisson, P. Larivee, P. P. McDonald, J. Stankova, and M. Rola-Pleszczynski
Signaling by the Cysteinyl-Leukotriene Receptor 2: INVOLVEMENT IN CHEMOKINE GENE TRANSCRIPTION
J. Biol. Chem., January 25, 2008; 283(4): 1974 - 1984.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Respir. Cell Mol. Bio.Home page
C. Thompson, A. Cloutier, Y. Bosse, M. Thivierge, C. L. Gouill, P. Larivee, P. P. McDonald, J. Stankova, and M. Rola-Pleszczynski
CysLT1 Receptor Engagement Induces Activator Protein-1- and NF-{kappa}B-Dependent IL-8 Expression
Am. J. Respir. Cell Mol. Biol., December 1, 2006; 35(6): 697 - 704.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Respir. Cell Mol. Bio.Home page
J. Zhu, Y.-S. Qiu, D. J. Figueroa, V. Bandi, H. Galczenski, K. Hamada, K. K. Guntupalli, J. F. Evans, and P. K. Jeffery
Localization and Upregulation of Cysteinyl Leukotriene-1 Receptor in Asthmatic Bronchial Mucosa
Am. J. Respir. Cell Mol. Biol., December 1, 2005; 33(6): 531 - 540.
[Abstract] [Full Text] [PDF]

Home page
 ChestHome page
W. A. Biernacki, S. A. Kharitonov, H. M. Biernacka, and P. J. Barnes
Effect of Montelukast on Exhaled Leukotrienes and Quality of Life in Asthmatic Patients
Chest, October 1, 2005; 128(4): 1958 - 1963.
[Abstract] [Full Text] [PDF]

Home page
 ChestHome page
W. Busse and M. Kraft
Cysteinyl Leukotrienes in Allergic Inflammation: Strategic Target for Therapy
Chest, April 1, 2005; 127(4): 1312 - 1326.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Respir. Crit. Care Med.Home page
K. Parameswaran, R. Watson, G. M. Gauvreau, R. Sehmi, and P. M. O'Byrne
The Effect of Pranlukast on Allergen-induced Bone Marrow Eosinophilopoiesis in Subjects with Asthma
Am. J. Respir. Crit. Care Med., April 15, 2004; 169(8): 915 - 920.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Respir. Crit. Care Med.Home page
F. R. Ali, W. L. G. Oldfield, N. Higashi, M. Larche, and A. B. Kay
Late Asthmatic Reactions Induced by Inhalation of Allergen-derived T Cell Peptides
Am. J. Respir. Crit. Care Med., January 1, 2004; 169(1): 20 - 26.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Respir. Cell Mol. Bio.Home page
Y. Cai, L. Bjermer, and T. S. Halstensen
Bronchial Mast Cells Are the Dominating LTC4S-Expressing Cells in Aspirin-Tolerant Asthma
Am. J. Respir. Cell Mol. Biol., December 1, 2003; 29(6): 683 - 693.
[Abstract] [Full Text] [PDF]

Home page
 ChestHome page
A. Sandrini, I. M. Ferreira, C. Gutierrez, J. R. Jardim, N. Zamel, and K. R. Chapman
Effect of Montelukast on Exhaled Nitric Oxide and Nonvolatile Markers of Inflammation in Mild Asthma
Chest, October 1, 2003; 124(4): 1334 - 1340.
[Abstract] [Full Text] [PDF]

Home page
 Eur Respir JHome page
G.F. Joos and B. O'Connor
Indirect airway challenges
Eur. Respir. J., June 1, 2003; 21(6): 1050 - 1068.
[Abstract] [Full Text] [PDF]

Home page
 ThoraxHome page
E Baraldi, S Carraro, R Alinovi, A Pesci, L Ghiro, A Bodini, G Piacentini, F Zacchello, and S Zanconato
Cysteinyl leukotrienes and 8-isoprostane in exhaled breath condensate of children with asthma exacerbations
Thorax, June 1, 2003; 58(6): 505 - 509.
[Abstract] [Full Text] [PDF]

Home page
 ThoraxHome page
R I Ketchell, M D'Amato, M W Jensen, and B J O'Connor
Contrasting effects of allergen challenge on airway responsiveness to cysteinyl leukotriene D4 and methacholine in mild asthma
Thorax, July 1, 2002; 57(7): 575 - 580.
[Abstract] [Full Text] [PDF]

Home page
 Eur Respir JHome page
Leader of the Working Group: M.M. Kelly, Members of the Working Group:, V. Keatings, R. Leigh, C. Peterson, J. Shute, P. Venge, and R. Djukanovic
Analysis of fluid{-}phase mediators
Eur. Respir. J., July 1, 2002; 20(37_suppl): 24S - 39s.
[Full Text] [PDF]

Home page
 Am. J. Respir. Crit. Care Med.Home page
S. Nag, B. Lamkhioued, and P. M. Renzi
Interleukin-2-induced Increased Airway Responsiveness and Lung Th2 Cytokine Expression Occur after Antigen Challenge through the Leukotriene Pathway
Am. J. Respir. Crit. Care Med., June 1, 2002; 165(11): 1540 - 1545.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Respir. Crit. Care Med.Home page
M. J. TOBIN
Asthma, Airway Biology, and Allergic Rhinitis in AJRCCM 2000
Am. J. Respir. Crit. Care Med., November 1, 2001; 164(9): 1559 - 1580.
[Full Text] [PDF]

Home page
 Am. J. Respir. Crit. Care Med.Home page
S. A. KHARITONOV and P. J. BARNES
Exhaled Markers of Pulmonary Disease
Am. J. Respir. Crit. Care Med., June 1, 2001; 163(7): 1693 - 1722.
[Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Proc. Am. Thorac. Soc. Am. J. Respir. Cell Mol. Biol.
Copyright © 2000 American Thoracic Society 		  ATS State of the Art 2009