Am. J. Respir. Crit. Care Med., Volume 164, Number 1, July 2001, 149-154

Decreased Pulmonary and Tracheal Smooth Muscle Expression and Activity of Type 1 Nitric Oxide Synthase (nNOS) after Ovalbumin Immunization and Multiple Aerosol Challenge in Guinea Pigs

ABDOULAYE SAMB, MARINA PRETOLANI, ANH-TUAN DINH-XUAN, HAKIMA OUKSEL, JACQUES CALLEBERT, CONSTANZA LISDERO, MICHEL AUBIER, and JORGE BOCZKOWSKI

Institut National de la Santé et de la Recherche Médicale (INSERM) U408 and IFR 02, Faculté X. Bichat; Service de Biochimie, Hôpital Lariboisière, Université Paris VII; Service de Physiologie-Explorations Fonctionnelles, Hôpital Cochin; and Assistance Publique-Hôpitaux de Paris, Université Paris V, Paris, France

Pharmacological evidence supports a role of a transient decreased endogenous nitric oxide (NO) synthesis in ovalbumin (OVA)-induced early airway hyperresponsiveness in guinea pigs. However, no data are available regarding the expression and activity of the constitutive NO synthases (cNOS; NOS1 and NOS3, nNOS and eNOS, respectively) in this model. Therefore, we evaluated cNOS activity (conversion of L-[³H]arginine to L-[³H]citrulline in the presence of Ca²⁺ and calmodulin), nitrate and nitrite (NOx) concentration (modified Griess method), and NOS1 and NOS3 protein expression (Western blot) in lung homogenates and in the tracheal smooth muscle from OVA-immunized and multiple aerosol-challenged guinea pigs (six challenges, once daily). The expression and activity of the inducible NOS isoform (NOS2), the levels of exhaled NO, and the in vivo airway reactivity were also determined. Constitutive NOS activity and NO_x concentration were significantly lower 6 h after the last OVA challenge as compared with saline exposure, being similar at 24 h. Expression of NOS1 paralleled cNOS activity, which was reduced 6, but not 24 h after OVA challenge. The decrease in NOS1 expression was accompanied by a significant decrease in the amounts of exhaled NO and by a maximal airway hyperresponsiveness to histamine. The levels of NOS3 were not modified at the two time points evaluated, and no NOS2 expression and activity were found at any time point. Similar modifications were observed in the tracheal smooth muscle. We conclude that OVA stimulation in immunized guinea pigs induced a transient reduction in NOS1 protein expression and activity in the respiratory system, which probably participates in airway hyperresponsiveness.

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