help button home button
AJRCCM
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Online Data Supplement
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by KRAMER, B. W.
Right arrow Articles by JOBE, A. H.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by KRAMER, B. W.
Right arrow Articles by JOBE, A. H.

Am. J. Respir. Crit. Care Med., Volume 164, Number 6, September 2001, 982-988

Dose and Time Response after Intraamniotic Endotoxin in Preterm Lambs

BORIS W. KRAMER, TIMOTHY J. MOSS, KAREN E. WILLET, JOHN P. NEWNHAM, PETER D. SLY, SUHAS G. KALLAPUR, MACHIKO IKEGAMI, and ALAN H. JOBE

Division of Pulmonary Biology, Children's Hospital Medical Center, Cincinnati, Ohio; and Department of Obstetrics and Gynecology and Division of Clinical Sciences, Center for Child Health Research at the University of Western Australia, Perth, Australia

Intraamniotic endotoxin causes chorioamnionitis, which is followed by improved fetal lung function after 4 d in fetal sheep. We evaluated 0.1 mg, 1 mg, 4 mg, and 10 mg endotoxin for inflammation and lung maturation effects after 7 d. Four and 10 mg endotoxin caused similar lung maturation and inflammation in the lung and chorioamnion. The number of neutrophils in cord blood and the inflammatory cells in alveolar lavage and fetal lung tissue increased in a dose-dependent manner. Lower endotoxin doses induced indicators of chorioamnionitis, lung and systemic inflammation without inducing lung maturation. Therefore, some degree of inflammation can occur without subsequent lung maturation. The inflammatory changes caused by 4 mg endotoxin were assessed after 5 h, 24 h, 72 h, and 7 d to discern local versus systemic inflammation after intraamniotic endotoxin. At 5 h active inflammatory cells were in the airways producing hydrogen peroxide, and interleukin-6 and -8 were increased in the cord blood indicating both lung and systemic responses. Cells recruited into the amniotic fluid produced proinflammatory cytokine mRNA for 7 d with no cytokine mRNA in chorioamnion, lung, or spleen after 72 h. The cells in the amniotic fluid may be a source of prolonged fetal exposure to proinflammatory cytokines.




This article has been cited by other articles:


Home page
 J. Immunol.Home page
S. G. Kallapur, A. H. Jobe, M. K. Ball, I. Nitsos, T. J. M. Moss, N. H. Hillman, J. P. Newnham, and B. W. Kramer
Pulmonary and Systemic Endotoxin Tolerance in Preterm Fetal Sheep Exposed to Chorioamnionitis
J. Immunol., December 15, 2007; 179(12): 8491 - 8499.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Respir. Crit. Care Med.Home page
N. H. Hillman, T. J. M. Moss, S. G. Kallapur, C. Bachurski, J. J. Pillow, G. R. Polglase, I. Nitsos, B. W. Kramer, and A. H. Jobe
Brief, Large Tidal Volume Ventilation Initiates Lung Injury and a Systemic Response in Fetal Sheep
Am. J. Respir. Crit. Care Med., September 15, 2007; 176(6): 575 - 581.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Lung Cell. Mol. Physiol.Home page
B. W. Kramer, S. N. Joshi, T. J. M. Moss, J. P. Newnham, R. Sindelar, A. H. Jobe, and S. G. Kallapur
Endotoxin-induced maturation of monocytes in preterm fetal sheep lung
Am J Physiol Lung Cell Mol Physiol, August 1, 2007; 293(2): L345 - L353.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Lung Cell. Mol. Physiol.Home page
S. Kunzmann, C. P. Speer, A. H. Jobe, and B. W. Kramer
Antenatal inflammation induced TGF-beta1 but suppressed CTGF in preterm lungs
Am J Physiol Lung Cell Mol Physiol, January 1, 2007; 292(1): L223 - L231.
[Abstract] [Full Text] [PDF]

Home page
 Reproductive SciencesHome page
I. Nitsos, S. M. Rees, J. Duncan, B. W. Kramer, R. Harding, J. P. Newnham, and T. J. M. Moss
Chronic Exposure to Intra-Amniotic Lipopolysaccharide Affects the Ovine Fetal Brain
Reproductive Sciences, May 1, 2006; 13(4): 239 - 247.
[Abstract] [PDF]

Home page
 Arch. Dis. Child. Fetal Neonatal Ed.Home page
S G Kallapur and A H Jobe
Contribution of inflammation to lung injury and development.
Arch. Dis. Child. Fetal Neonatal Ed., March 1, 2006; 91(2): F132 - F135.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Respir. Crit. Care Med.Home page
S. G. Kallapur, T. J. M. Moss, M. Ikegami, R. L. Jasman, J. P. Newnham, and A. H. Jobe
Recruited Inflammatory Cells Mediate Endotoxin-induced Lung Maturation in Preterm Fetal Lambs
Am. J. Respir. Crit. Care Med., November 15, 2005; 172(10): 1315 - 1321.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Lung Cell. Mol. Physiol.Home page
S. G. Kallapur, I. Nitsos, T. J. M. Moss, B. W. Kramer, J. P. Newnham, M. Ikegami, and A. H. Jobe
Chronic endotoxin exposure does not cause sustained structural abnormalities in the fetal sheep lungs
Am J Physiol Lung Cell Mol Physiol, May 1, 2005; 288(5): L966 - L974.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Respir. Crit. Care Med.Home page
B. W. Kramer, M. Ikegami, T. J. M. Moss, I. Nitsos, J. P. Newnham, and A. H. Jobe
Endotoxin-induced Chorioamnionitis Modulates Innate Immunity of Monocytes in Preterm Sheep
Am. J. Respir. Crit. Care Med., January 1, 2005; 171(1): 73 - 77.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Lung Cell. Mol. Physiol.Home page
S. G. Kallapur, C. J. Bachurski, T. D. L. Cras, S. N. Joshi, M. Ikegami, and A. H. Jobe
Vascular changes after intra-amniotic endotoxin in preterm lamb lungs
Am J Physiol Lung Cell Mol Physiol, December 1, 2004; 287(6): L1178 - L1185.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Lung Cell. Mol. Physiol.Home page
S. C. Land and F. Darakhshan
Thymulin evokes IL-6-C/EBP{beta} regenerative repair and TNF-{alpha} silencing during endotoxin exposure in fetal lung explants
Am J Physiol Lung Cell Mol Physiol, March 1, 2004; 286(3): L473 - L487.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Lung Cell. Mol. Physiol.Home page
M. Ikegami, S. G. Kallapur, and A. H. Jobe
Initial responses to ventilation of premature lambs exposed to intra-amniotic endotoxin 4 days before delivery
Am J Physiol Lung Cell Mol Physiol, March 1, 2004; 286(3): L573 - L579.
[Abstract] [Full Text] [PDF]

Home page
 Cardiovasc ResHome page
S. Rounioja, J. Rasanen, V. Glumoff, M. Ojaniemi, K. Makikallio, and M. Hallman
Intra-amniotic lipopolysaccharide leads to fetal cardiac dysfunction: A mouse model for fetal inflammatory response
Cardiovasc Res, October 15, 2003; 60(1): 156 - 164.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Lung Cell. Mol. Physiol.Home page
M. Ikegami, T. J. M. Moss, S. G. Kallapur, N. Mulrooney, B. W. Kramer, I. Nitsos, C. J. Bachurski, J. P. Newnham, and A. H. Jobe
Minimal lung and systemic responses to TNF-{alpha} in preterm sheep
Am J Physiol Lung Cell Mol Physiol, July 1, 2003; 285(1): L121 - L129.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Lung Cell. Mol. Physiol.Home page
S. G. Kallapur, B. W. Kramer, T. J. M. Moss, J. P. Newnham, A. H. Jobe, M. Ikegami, and C. J. Bachurski
Maternal glucocorticoids increase endotoxin-induced lung inflammation in preterm lambs
Am J Physiol Lung Cell Mol Physiol, April 1, 2003; 284(4): L633 - L642.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Respir. Crit. Care Med.Home page
S. G. Kallapur, A. H. Jobe, M. Ikegami, and C. J. Bachurski
Increased IP-10 and MIG Expression after Intra-amniotic Endotoxin in Preterm Lamb Lung
Am. J. Respir. Crit. Care Med., March 1, 2003; 167(5): 779 - 786.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Lung Cell. Mol. Physiol.Home page
B. W. Kramer, S. Kramer, M. Ikegami, and A. H. Jobe
Injury, inflammation, and remodeling in fetal sheep lung after intra-amniotic endotoxin
Am J Physiol Lung Cell Mol Physiol, August 1, 2002; 283(2): L452 - L459.
[Abstract] [Full Text] [PDF]

Home page
 Reproductive SciencesHome page
T. J. M. Moss, M. G. Davey, R. Harding, and J. P. Newnham
Effects of Intra-Amniotic Endotoxin on Lung Structure and Function Two Months After Term Birth in Sheep
Reproductive Sciences, July 1, 2002; 9(4): 220 - 225.
[Abstract] [PDF]

Home page
 Am. J. Respir. Crit. Care Med.Home page
D. Warburton
Sound the Tocsin! Beware Adverse Effects of Lung Inflammation Early in Gestation
Am. J. Respir. Crit. Care Med., March 15, 2002; 165(6): 741 - 742.
[Full Text] [PDF]

Home page
 Reproductive SciencesHome page
I. Nitsos, T. J. M. Moss, M. L. Cock, R. Harding, and J. P. Newnham
Fetal Responses to Intra-Amniotic Endotoxin in Sheep
Reproductive Sciences, March 1, 2002; 9(2): 80 - 85.
[Abstract] [PDF]

Home page
 Am. J. Respir. Crit. Care Med.Home page
M. J. TOBIN
Pediatrics, Surfactant, and Cystic Fibrosis in AJRCCM 2001
Am. J. Respir. Crit. Care Med., March 1, 2002; 165(5): 619 - 630.
[Full Text] [PDF]

Home page
 Am. J. Respir. Crit. Care Med.Home page
B. W. KRAMER, M. IKEGAMI, and A. H. JOBE
Intratracheal Endotoxin Causes Systemic Inflammation in Ventilated Preterm Lambs
Am. J. Respir. Crit. Care Med., February 15, 2002; 165(4): 463 - 469.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Proc. Am. Thorac. Soc. Am. J. Respir. Cell Mol. Biol.
Copyright © 2001 American Thoracic Society 		  ATS State of the Art 2009