Published ahead of print on February 28, 2008, doi:10.1164/rccm.200710-1458OC
© 2008 American Thoracic Society doi: 10.1164/rccm.200710-1458OC
Circulating Endothelial Microparticle Levels Predict Hemodynamic Severity of Pulmonary Hypertension1 Division of Cardiology, Department of Medicine, University of California, San Francisco, San Francisco, California Correspondence and requests for reprints should be addressed to Yerem Yeghiazarians, M.D., F.A.C.C., F.A.H.A., F.S.C.A.I., Division of Cardiology, University of California, San Francisco, 505 Parnassus Avenue, San Francisco, CA 94143-0103. E-mail: yeghiaza{at}medicine.ucsf.edu Rationale: Circulating microparticles (MPs) are submicron membrane fragments shed from damaged or activated vascular cells. Endothelial MPs are a biological marker of dysfunctional endothelium. Vascular remodeling and endothelial dysfunction are involved in pulmonary hypertension (PH). Objectives: We tested the hypothesis that circulating MPs are increased in patients with PH and that identifiable subgroups of MPs predict the hemodynamic severity of this condition progression. Methods: Patients (n = 24; age, 54 ± 4 yr) undergoing right heart catheterization for precapillary PH without any endothelium-active vasodilator therapy participated in the study. Age- and sex-matched healthy control subjects (n = 20) were included. Endothelial (PECAM+ [CD31+]/ CD41–, VE-cadherin+ [CD144+], and E-selectin+ [CD62e+]), platelet (CD41+), leukocyte-derived (CD45+), and annexin V+ MPs were measured by flow cytometry in platelet-free plasma from venous blood. Measurements and Main Results: Levels of circulating endothelial PECAM+, VE-cadherin+, E-selectin+, and leukocyte-derived MPs, but not platelet and annexin V+ MPs, were increased in subjects with PH compared with control subjects (P < 0.01 each). PECAM+ and VE-cadherin+ MP levels significantly correlated with mean pulmonary artery pressure (r = 0.92 and r = 0.87, respectively), pulmonary vascular resistance (r = 0.78 and r = 0.73), and mean right atrial pressure (r = 0.43, and r = 0.46) and correlated inversely with cardiac index (r = –0.59 and r = –0.52). These relationships were not observed for other MP subgroups, and persisted in multivariate analysis after adjustment for confounding factors. Conclusions: In subjects with precapillary PH, levels of circulating endothelial and leukocyte MPs were increased compared with control subjects. In addition, levels of PECAM+ and VE-cadherin+, but not E-selectin+, endothelial MPs predicted hemodynamic severity of the disease.
Key Words: pulmonary hypertension • endothelium • microparticles • hemodynamics
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